Asian Journal of Pharmaceutical Analysis

Pankaj Savani ¹, Sudhanshu Chauhan ¹, Vineet Jain ², Hasumati Raj ¹, Sagar Patel ¹

Affiliations
1 Department of Quality Assurance, Shree Dhanvantary Pharmacy College, Kim, Dist: Surat, Gujarat 394110, IN
2 Department of Pharmacognosy, Shree Dhanvantary Pharmacy College, Kim, Dist: Surat, Gujarat 394110, IN

Abstract

A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Clopidogrel Bisulphate and Irbesartan in synthetic mixture using simultaneous equation Method. In this spectroscopic method, for Clopidogrel Bisulphate 220.00 nm and 250.00 nm wavelengths were selected for measurement of absorptivity. Both the drugs show linearity in a concentration range of 10-50 μg/ml at their respective λmax with correlation coefficient (r2) of 0.9996 and 0.9998 for Clopidogrel Bisulphate and Irbesartan, respectively. Accuracy, precision and recovery studies were done by QC samples covering lower, medium and high concentrations of the linearity range. The relative standard deviation for accuracy, precision studies were found to be within the acceptance range (< 2%). The limit of determination was 0.243 μg/ml and 0.323 μg/ml for Clopidogrel Bisulphate and Irbesartan, respectively. The limit of quantification was 0.737 μg/ml and 0.980 μg/ml for Clopidogrel Bisulphate and Irbesartan, respectively. Recovery of Clopidogrel Bisulphate and Irbesartan were found to be 99.57 % and 99.68 % respectively confirming the accuracy of the proposed method. % Assay was found to be 99.41 % and 99.22 % for Clopidogrel Bisulphate and Irbesartan, respectively. The proposed method is recommended for routine analysis since they are rapid, simple, accurate and also sensitive and specific by no heating and no organic solvent extraction.

Keywords

Clopidogrel Bisulphate, Irbesartan, Simultaneous Estimation, Validation Method.

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Sudhanshu I. Chauhan ¹, Pankaj D. Savani ¹, Hasumati Raj ¹, Sagar Patel ¹

Affiliations
1 Department of Quality Assurance, Shree Dhanvantary Pharmacy College, Kim, Surat, Gujarat, IN

Abstract

Candesartan Cilexetil is classified as an AT1 Angiotensin II receptor antagonist as a Antihypertensive agent. There are widely used in treatment of diseases like hypertension, heart failure, myocardial infarction and diabetic nephropathy. The clinical and pharmaceutical analysis of this drug requires effective analytical procedures for quality control and Pharmcodynamic and pharmacokinetic studies as well as stability study. There are many article examines published analytical methods reported so far in the literature for the determination of Candesartan Cilexetil in biological samples and pharmaceutical formulations. These article comprise reviews of analytical methods like Spectrophotometric methods, Chromatographic method including HPLC, HPTLC.

Keywords

Candesartan Cilexetil, Spectrophotometry, HPLC, Anti-Hypertensive Agent, AT1 Angiotensin II Receptor Antagonist.

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